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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- V2001 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Smooth Muscle Actin Antibody
- Antibody type
- Monoclonal
- Antigen
- An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Feline, Goat, Guinea Pig, Porcine, Rabbit, Sheep, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1A4
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).
Submitted references The P2Y2 nucleotide receptor is an inhibitor of vascular calcification.
Pathological interstitial vascular proliferation adjacent to glomeruli in immunoglobulin a nephropathy.
Cancer-associated fibroblast suppresses killing activity of natural killer cells through downregulation of poliovirus receptor (PVR/CD155), a ligand of activating NK receptor.
Immunohistochemical localization of alpha and beta adrenergic receptors in the human nasal turbinate.
p27(Kip1) and p21(Cip1)-independent proliferative inhibition of vascular smooth muscle cells cultured in type-I collagen matrix honeycombs.
Sex Dimorphism Profile of Alzheimer's Disease-Type Pathologies in an APP/PS1 Mouse Model.
Use of a Barbed Knotless Suture for Laparoscopic Ablation of the Nephrosplenic Space in 8 Horses.
Peripheral Levels of AGEs and Astrocyte Alterations in the Hippocampus of STZ-Diabetic Rats.
Lacrimal Gland Repair Using Progenitor Cells.
IL-33 is related to innate immune activation and sensitization to HDM in mild steroid-free asthma.
Intrinsic Deregulation of Vascular Smooth Muscle and Myofibroblast Differentiation in Mesenchymal Stromal Cells from Patients with Systemic Sclerosis.
Notch ligand Delta-like 1 promotes in vivo vasculogenesis in human cord blood-derived endothelial colony forming cells.
Myofibroblasts in proliferative diabetic retinopathy can originate from infiltrating fibrocytes and through endothelial-to-mesenchymal transition (EndoMT).
Histopathological characterization of renal tubular and interstitial changes in 5/6 nephrectomized marmoset monkeys (Callithrix jacchus).
Stromal myofibroblasts in potentially malignant and malignant lesions of the oral cavity.
Histological analysis of the structural composition of ankle ligaments.
Glycated Collagen Induces α11 Integrin Expression Through TGF-β2 and Smad3.
Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling.
Epidemiologic, Clinicopathological, Immunohistochemical and Molecular Analysis of Gastrointestinal Glomus Tumors
Polymeric system for dual growth factor delivery.
Smooth muscle cell phenotypic transition associated with calcification: upregulation of Cbfa1 and downregulation of smooth muscle lineage markers.
Dependence of collagen remodelling on alpha-smooth muscle actin expression by fibroblasts.
Qian S, Regan JN, Shelton MT, Hoggatt A, Mohammad KS, Herring PB, Seye CI
Atherosclerosis 2017 Feb;257:38-46
Atherosclerosis 2017 Feb;257:38-46
Pathological interstitial vascular proliferation adjacent to glomeruli in immunoglobulin a nephropathy.
Mori H, Nishi S, Ueno M, Imai N, Ookawara S, Mirishita Y, Narita I, Tabei K
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia 2016 Sep-Oct;27(5):885-892
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia 2016 Sep-Oct;27(5):885-892
Cancer-associated fibroblast suppresses killing activity of natural killer cells through downregulation of poliovirus receptor (PVR/CD155), a ligand of activating NK receptor.
Inoue T, Adachi K, Kawana K, Taguchi A, Nagamatsu T, Fujimoto A, Tomio K, Yamashita A, Eguchi S, Nishida H, Nakamura H, Sato M, Yoshida M, Arimoto T, Wada-Hiraike O, Oda K, Osuga Y, Fujii T
International journal of oncology 2016 Oct;49(4):1297-304
International journal of oncology 2016 Oct;49(4):1297-304
Immunohistochemical localization of alpha and beta adrenergic receptors in the human nasal turbinate.
Shirasaki H, Kanaizumi E, Himi T
Auris, nasus, larynx 2016 Jun;43(3):309-14
Auris, nasus, larynx 2016 Jun;43(3):309-14
p27(Kip1) and p21(Cip1)-independent proliferative inhibition of vascular smooth muscle cells cultured in type-I collagen matrix honeycombs.
Uchida M, Suzuki S, Suzuki T, Ishii I
Microvascular research 2016 Jan;103:36-40
Microvascular research 2016 Jan;103:36-40
Sex Dimorphism Profile of Alzheimer's Disease-Type Pathologies in an APP/PS1 Mouse Model.
Jiao SS, Bu XL, Liu YH, Zhu C, Wang QH, Shen LL, Liu CH, Wang YR, Yao XQ, Wang YJ
Neurotoxicity research 2016 Feb;29(2):256-66
Neurotoxicity research 2016 Feb;29(2):256-66
Use of a Barbed Knotless Suture for Laparoscopic Ablation of the Nephrosplenic Space in 8 Horses.
Albanese V, Hanson RR, McMaster MA, Koehler JW, Caldwell FJ
Veterinary surgery : VS 2016 Aug;45(6):824-30
Veterinary surgery : VS 2016 Aug;45(6):824-30
Peripheral Levels of AGEs and Astrocyte Alterations in the Hippocampus of STZ-Diabetic Rats.
Nardin P, Zanotto C, Hansen F, Batassini C, Gasparin MS, Sesterheim P, Gonçalves CA
Neurochemical research 2016 Aug;41(8):2006-16
Neurochemical research 2016 Aug;41(8):2006-16
Lacrimal Gland Repair Using Progenitor Cells.
Gromova A, Voronov DA, Yoshida M, Thotakura S, Meech R, Dartt DA, Makarenkova HP
Stem cells translational medicine 2016 Aug 15;
Stem cells translational medicine 2016 Aug 15;
IL-33 is related to innate immune activation and sensitization to HDM in mild steroid-free asthma.
Porsbjerg C, Baines K, Gibson P, Bergqvist A, Erjefält JS, Sverrild A, Backer V
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2016 Apr;46(4):564-74
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2016 Apr;46(4):564-74
Intrinsic Deregulation of Vascular Smooth Muscle and Myofibroblast Differentiation in Mesenchymal Stromal Cells from Patients with Systemic Sclerosis.
Hegner B, Schaub T, Catar R, Kusch A, Wagner P, Essin K, Lange C, Riemekasten G, Dragun D
PloS one 2016 Apr 7;11(4):e0153101
PloS one 2016 Apr 7;11(4):e0153101
Notch ligand Delta-like 1 promotes in vivo vasculogenesis in human cord blood-derived endothelial colony forming cells.
Kim H, Huang L, Critser PJ, Yang Z, Chan RJ, Wang L, Carlesso N, Voytik-Harbin SL, Bernstein ID, Yoder MC
Cytotherapy 2015 May;17(5):579-92
Cytotherapy 2015 May;17(5):579-92
Myofibroblasts in proliferative diabetic retinopathy can originate from infiltrating fibrocytes and through endothelial-to-mesenchymal transition (EndoMT).
Abu El-Asrar AM, De Hertogh G, van den Eynde K, Alam K, Van Raemdonck K, Opdenakker G, Van Damme J, Geboes K, Struyf S
Experimental eye research 2015 Mar;132:179-89
Experimental eye research 2015 Mar;132:179-89
Histopathological characterization of renal tubular and interstitial changes in 5/6 nephrectomized marmoset monkeys (Callithrix jacchus).
Suzuki Y, Yamaguchi I, Myojo K, Kimoto N, Imaizumi M, Takada C, Sanada H, Takaba K, Yamate J
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2015 Jan;67(1):41-51
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2015 Jan;67(1):41-51
Stromal myofibroblasts in potentially malignant and malignant lesions of the oral cavity.
Rodrigues PC, DA Costa Miguel MC, DE Aquino SN, Fonseca FP, Silva AR, Leme AF, Coletta RD
Oncology letters 2015 Feb;9(2):667-670
Oncology letters 2015 Feb;9(2):667-670
Histological analysis of the structural composition of ankle ligaments.
Rein S, Hagert E, Schneiders W, Fieguth A, Zwipp H
Foot & ankle international 2015 Feb;36(2):211-24
Foot & ankle international 2015 Feb;36(2):211-24
Glycated Collagen Induces α11 Integrin Expression Through TGF-β2 and Smad3.
Talior-Volodarsky I, Arora PD, Wang Y, Zeltz C, Connelly KA, Gullberg D, McCulloch CA
Journal of cellular physiology 2015 Feb;230(2):327-36
Journal of cellular physiology 2015 Feb;230(2):327-36
Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling.
Lian X, Bao X, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP
Stem cell reports 2014 Nov 11;3(5):804-16
Stem cell reports 2014 Nov 11;3(5):804-16
Epidemiologic, Clinicopathological, Immunohistochemical and Molecular Analysis of Gastrointestinal Glomus Tumors
Nosseir S
Journal of Clinical & Experimental Pathology 2014 ;04(05)
Journal of Clinical & Experimental Pathology 2014 ;04(05)
Polymeric system for dual growth factor delivery.
Richardson TP, Peters MC, Ennett AB, Mooney DJ
Nature biotechnology 2001 Nov;19(11):1029-34
Nature biotechnology 2001 Nov;19(11):1029-34
Smooth muscle cell phenotypic transition associated with calcification: upregulation of Cbfa1 and downregulation of smooth muscle lineage markers.
Steitz SA, Speer MY, Curinga G, Yang HY, Haynes P, Aebersold R, Schinke T, Karsenty G, Giachelli CM
Circulation research 2001 Dec 7;89(12):1147-54
Circulation research 2001 Dec 7;89(12):1147-54
Dependence of collagen remodelling on alpha-smooth muscle actin expression by fibroblasts.
Arora PD, McCulloch CA
Journal of cellular physiology 1994 Apr;159(1):161-75
Journal of cellular physiology 1994 Apr;159(1):161-75
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human uterus lysate with Smooth muscle actin antibody (clone 1A4).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: human leiomyosarcoma stained with Smooth muscle actin antibody (1A4).
