Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
Reference
Comment
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- Product number
- V2318 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Muscle Actin Antibody
- Antibody type
- Monoclonal
- Antigen
- SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- HHF35
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Anti-inflammatory role of obestatin in autoimmune myocarditis.
The Spindle Cell Neoplasms of the Oral Cavity.
Reactive Nodular Fibrous Pseudotumor Presenting as a Huge Intra abdominal Mass after Abdominal Surgery: a Case Report.
High-grade myofibroblastic sarcoma of the mandible: case report in a child and literature review
Primary sinonasal mucosal melanoma with aberrant diffuse and strong desmin reactivity: a potential diagnostic pitfall!
Desmoplastic fibroma of the ulna bone.
Primary leiomyosarcoma of the abdominal wall mimicking nodular fasciitis in a child
Comparison of the expression of vimentin and actin in spitz nevi and spitzoid malignant melanomas.
Hepatic angiomyolipomas: ultrasonic characteristics of 25 patients from a single center.
Diffuse Large B-Cell Lymphoma: Case Report
Primary renal myxofibrosarcoma.
Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells.
Spindle cell sarcoma of the vulva with myofibroblastic differentiation.
Solitary myofibroma of the mandible: an immunohistochemical and ultrastructural study with a review of the literature.
Primary peritoneal angiosarcoma: a case report.
Rhabdomyosarcoma of the duodenum: report of a case.
Complete pathological response in advanced extra-gastrointestinal stromal tumor after imatinib mesylate therapy: a case report.
HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues.
HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.
Pamukcu O, Baykan A, Bayram LC, Narin F, Cetin N, Narin N, Argun M, Ozyurt A, Uzum K
Clinical and experimental pharmacology & physiology 2016 Jan;43(1):47-55
Clinical and experimental pharmacology & physiology 2016 Jan;43(1):47-55
The Spindle Cell Neoplasms of the Oral Cavity.
Shamim T
Iranian journal of pathology 2015 Summer;10(3):175-84
Iranian journal of pathology 2015 Summer;10(3):175-84
Reactive Nodular Fibrous Pseudotumor Presenting as a Huge Intra abdominal Mass after Abdominal Surgery: a Case Report.
Ciftci B, Vardar E, Tasli F, Yakan S, Top E, Yildirim M
Iranian journal of pathology 2015 Spring;10(2):149-54
Iranian journal of pathology 2015 Spring;10(2):149-54
High-grade myofibroblastic sarcoma of the mandible: case report in a child and literature review
de Albuquerque R, Melo S, Bastos T, Rocha R, Piva M, de Melo M
Oral Surgery 2015 May;8(2):91-95
Oral Surgery 2015 May;8(2):91-95
Primary sinonasal mucosal melanoma with aberrant diffuse and strong desmin reactivity: a potential diagnostic pitfall!
Smith SM, Schmitt AC, Carrau RL, Iwenofu OH
Head and neck pathology 2015 Mar;9(1):165-71
Head and neck pathology 2015 Mar;9(1):165-71
Desmoplastic fibroma of the ulna bone.
Eyesan SU, Kehinde TG, Adesina AS, Ayeni CS, Abdulkareem BF
Nigerian medical journal : journal of the Nigeria Medical Association 2015 Mar-Apr;56(2):153-5
Nigerian medical journal : journal of the Nigeria Medical Association 2015 Mar-Apr;56(2):153-5
Primary leiomyosarcoma of the abdominal wall mimicking nodular fasciitis in a child
Koike Y, Imaoka H, Otake K, Inoue M, Uchida K, Kusunoki M
Journal of Pediatric Surgery Case Reports 2015 June;3(6):252-254
Journal of Pediatric Surgery Case Reports 2015 June;3(6):252-254
Comparison of the expression of vimentin and actin in spitz nevi and spitzoid malignant melanomas.
Alomari AK, Klump V, Neumeister V, Ariyan S, Narayan D, Lazova R
The American Journal of dermatopathology 2015 Jan;37(1):46-51
The American Journal of dermatopathology 2015 Jan;37(1):46-51
Hepatic angiomyolipomas: ultrasonic characteristics of 25 patients from a single center.
Wang B, Ye Z, Chen Y, Zhao Q, Huang M, Chen F, Li Y, Jiang T
Ultrasound in medicine & biology 2015 Feb;41(2):393-400
Ultrasound in medicine & biology 2015 Feb;41(2):393-400
Diffuse Large B-Cell Lymphoma: Case Report
FERNANDES D, BUFALINO A, VARGAS P, DE ALMEIDA O, NAVARRO C, MASSUCATO E, ONOFRE M
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2015 August;120(2)
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2015 August;120(2)
Primary renal myxofibrosarcoma.
Val-Bernal JF, García-González MR, Mayorga M, Marrero RH, Jorge-Pérez N
Pathology, research and practice 2015 Aug;211(8):619-24
Pathology, research and practice 2015 Aug;211(8):619-24
Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells.
Ma L, Zhu XF, Wu YY, Chen KJ, Shi DZ, Yin HJ
Chinese journal of integrative medicine 2015 Apr;21(4):299-306
Chinese journal of integrative medicine 2015 Apr;21(4):299-306
Spindle cell sarcoma of the vulva with myofibroblastic differentiation.
Adeleye AJ, Palmeri N, Wang SH, Liu-Jarin X, Wright JD
Journal of lower genital tract disease 2015 Apr;19(2):e38-9
Journal of lower genital tract disease 2015 Apr;19(2):e38-9
Solitary myofibroma of the mandible: an immunohistochemical and ultrastructural study with a review of the literature.
Satomi T, Kohno M, Enomoto A, Abukawa H, Fujikawa K, Koizumi T, Chikazu D, Matsubayashi J, Nagao T
Medical molecular morphology 2014 Sep;47(3):176-83
Medical molecular morphology 2014 Sep;47(3):176-83
Primary peritoneal angiosarcoma: a case report.
Litvan J, Aghazarian M, Wiley E, Guleria S, Dudek AZ
Anticancer research 2014 Sep;34(9):5001-6
Anticancer research 2014 Sep;34(9):5001-6
Rhabdomyosarcoma of the duodenum: report of a case.
Sato A, Hashimoto M, Moriyama J, Hanaoka M, Momose K, Kinowaki K, Ando S, Fujii T, Ohashi K, Watanabe G
Surgery today 2014 Feb;44(2):378-82
Surgery today 2014 Feb;44(2):378-82
Complete pathological response in advanced extra-gastrointestinal stromal tumor after imatinib mesylate therapy: a case report.
Rediti M, Pellegrini E, Molinara E, Cerullo C, Fonte C, Lunghi A, Iori A, Neri B
Anticancer research 2014 Feb;34(2):905-7
Anticancer research 2014 Feb;34(2):905-7
HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues.
Tsukada T, McNutt MA, Ross R, Gown AM
The American journal of pathology 1987 May;127(2):389-402
The American journal of pathology 1987 May;127(2):389-402
HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.
Tsukada T, Tippens D, Gordon D, Ross R, Gown AM
The American journal of pathology 1987 Jan;126(1):51-60
The American journal of pathology 1987 Jan;126(1):51-60
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of human leiomyosarcoma (10X) with Muscle actin antibody (HHF35).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of human leiomyosarcoma (20X) with Muscle actin antibody (HHF35).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Muscle Actin Antibody (clone HHF35). Confirmation of Integrity and Purity of the Antibody.