V2318

antibody from NSJ Bioreagents

Targeting: ACTA2 ACTSA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2318

Provider

NSJ Bioreagents

Product name

Muscle Actin Antibody

Antibody type

Monoclonal

Antigen

SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.

Description

Protein G purified antibody

Reactivity

Human, Rabbit

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

HHF35

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Anti-inflammatory role of obestatin in autoimmune myocarditis.

Pamukcu O, Baykan A, Bayram LC, Narin F, Cetin N, Narin N, Argun M, Ozyurt A, Uzum K
Clinical and experimental pharmacology & physiology 2016 Jan;43(1):47-55

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The Spindle Cell Neoplasms of the Oral Cavity.

Shamim T
Iranian journal of pathology 2015 Summer;10(3):175-84

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Reactive Nodular Fibrous Pseudotumor Presenting as a Huge Intra abdominal Mass after Abdominal Surgery: a Case Report.

Ciftci B, Vardar E, Tasli F, Yakan S, Top E, Yildirim M
Iranian journal of pathology 2015 Spring;10(2):149-54

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High-grade myofibroblastic sarcoma of the mandible: case report in a child and literature review

de Albuquerque R, Melo S, Bastos T, Rocha R, Piva M, de Melo M
Oral Surgery 2015 May;8(2):91-95

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Primary sinonasal mucosal melanoma with aberrant diffuse and strong desmin reactivity: a potential diagnostic pitfall!

Smith SM, Schmitt AC, Carrau RL, Iwenofu OH
Head and neck pathology 2015 Mar;9(1):165-71

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Desmoplastic fibroma of the ulna bone.

Eyesan SU, Kehinde TG, Adesina AS, Ayeni CS, Abdulkareem BF
Nigerian medical journal : journal of the Nigeria Medical Association 2015 Mar-Apr;56(2):153-5

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Primary leiomyosarcoma of the abdominal wall mimicking nodular fasciitis in a child

Koike Y, Imaoka H, Otake K, Inoue M, Uchida K, Kusunoki M
Journal of Pediatric Surgery Case Reports 2015 June;3(6):252-254

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Comparison of the expression of vimentin and actin in spitz nevi and spitzoid malignant melanomas.

Alomari AK, Klump V, Neumeister V, Ariyan S, Narayan D, Lazova R
The American Journal of dermatopathology 2015 Jan;37(1):46-51

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Hepatic angiomyolipomas: ultrasonic characteristics of 25 patients from a single center.

Wang B, Ye Z, Chen Y, Zhao Q, Huang M, Chen F, Li Y, Jiang T
Ultrasound in medicine & biology 2015 Feb;41(2):393-400

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Diffuse Large B-Cell Lymphoma: Case Report

FERNANDES D, BUFALINO A, VARGAS P, DE ALMEIDA O, NAVARRO C, MASSUCATO E, ONOFRE M
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 2015 August;120(2)

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Primary renal myxofibrosarcoma.

Val-Bernal JF, García-González MR, Mayorga M, Marrero RH, Jorge-Pérez N
Pathology, research and practice 2015 Aug;211(8):619-24

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Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells.

Ma L, Zhu XF, Wu YY, Chen KJ, Shi DZ, Yin HJ
Chinese journal of integrative medicine 2015 Apr;21(4):299-306

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Spindle cell sarcoma of the vulva with myofibroblastic differentiation.

Adeleye AJ, Palmeri N, Wang SH, Liu-Jarin X, Wright JD
Journal of lower genital tract disease 2015 Apr;19(2):e38-9

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Solitary myofibroma of the mandible: an immunohistochemical and ultrastructural study with a review of the literature.

Satomi T, Kohno M, Enomoto A, Abukawa H, Fujikawa K, Koizumi T, Chikazu D, Matsubayashi J, Nagao T
Medical molecular morphology 2014 Sep;47(3):176-83

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Primary peritoneal angiosarcoma: a case report.

Litvan J, Aghazarian M, Wiley E, Guleria S, Dudek AZ
Anticancer research 2014 Sep;34(9):5001-6

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Rhabdomyosarcoma of the duodenum: report of a case.

Sato A, Hashimoto M, Moriyama J, Hanaoka M, Momose K, Kinowaki K, Ando S, Fujii T, Ohashi K, Watanabe G
Surgery today 2014 Feb;44(2):378-82

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Complete pathological response in advanced extra-gastrointestinal stromal tumor after imatinib mesylate therapy: a case report.

Rediti M, Pellegrini E, Molinara E, Cerullo C, Fonte C, Lunghi A, Iori A, Neri B
Anticancer research 2014 Feb;34(2):905-7

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HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues.

Tsukada T, McNutt MA, Ross R, Gown AM
The American journal of pathology 1987 May;127(2):389-402

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HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.

Tsukada T, Tippens D, Gordon D, Ross R, Gown AM
The American journal of pathology 1987 Jan;126(1):51-60

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of human leiomyosarcoma (10X) with Muscle actin antibody (HHF35).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of human leiomyosarcoma (20X) with Muscle actin antibody (HHF35).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Muscle Actin Antibody (clone HHF35). Confirmation of Integrity and Purity of the Antibody.