Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- STJ97811 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ACTA2 antibody [1H8] (STJ97811)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Actin-Aortic Smooth Muscle is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry and ELISA research applications.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis using ACTA2 monoclonal antibody against HeLa (1) , Jurkta (2) , HepG2 (3) , MCF-7 (4) , A431 (5) , A549 (6) , PC-12 (7) , NIH/3T3 (8) and Cos7 (9) cell lysate.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using ACTA2 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using ACTA2 monoclonal antibody (green) and negative control (purple).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA