Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00009973-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009973-M03, RRID:AB_1724791
- Product name
- Mouse Monoclonal CCS/SOD4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Reacts with copper chaperone for superoxide dismutase.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Impaired copper and iron metabolism in blood cells and muscles of patients affected by copper deficiency myeloneuropathy.
Spinazzi M, Sghirlanzoni A, Salviati L, Angelini C
Neuropathology and applied neurobiology 2014 Dec;40(7):888-98
Neuropathology and applied neurobiology 2014 Dec;40(7):888-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Analysis of CCS expression in transfected 293T cell line by CCS monoclonal antibody (M03), clone 1E2. Lane 1: CCS transfected lysate (Predicted MW: 29 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Detection limit for recombinant GST tagged CCS is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Analysis of CCS transfected lysate using anti-CCS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CCS monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Analysis of monoclonal antibody to CCS on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1.5 ug/ml