16034-1-AP

antibody from Invitrogen Antibodies

Targeting: PSMD7 MOV34, P40, Rpn8, S12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16034-1-AP

Provider

Invitrogen Antibodies

Product name

PSMD7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPELAVQKV VVHPLVLLSV VDHFNRIGKV GNQKRVVGVL LGSWQKKVLD VSNSFAVPFD EDDKDDSVWF LDHDYLENMY GMFKKVNARE RIVGWYHTGP KLHKNDIAIN ELMKRYCPNS VLVIIDVKPK DLGLPTEAYI SVEEVHDDGT PTSKTFEHVT SEIGAEEAEE VGVEHLLRDI KDTTVGTLSQ RITNQVHGLK GLNSKLLDIR SYLEKVATGK LPINHQIIYQ LQDVFNLLPD VSLQEFVKAF YLKTNDQMVV VYLASLIRSV VALHNLINNK IANRDAEKKE GQEKEESKKD RKEDKEKDKD KEKSDVKKEE (1-319 aa encoded by BC012606)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16034-1-AP ( PSMD7 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug PSMD7 antibody (16034-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PSMD7 (IP:16034-1-AP, 3ug; Detection:16034-1-AP 1:500) with K-562 cells lysate 2400ug.