PA5-27346

antibody from Invitrogen Antibodies

Targeting: LIPA CESD, LAL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27346

Provider

Invitrogen Antibodies

Product name

LIPA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, A549, H1299, HCT116, Mouse liver. Predicted reactivity: Pig (83%), Rhesus Monkey (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.57 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8(+) T cells at the expense of central memory.

Cannons JL, Villarino AV, Kapnick SM, Preite S, Shih HY, Gomez-Rodriguez J, Kaul Z, Shibata H, Reilley JM, Huang B, Handon R, McBain IT, Gossa S, Wu T, Su HC, McGavern DB, O'Shea JJ, McGuire PJ, Uzel G, Schwartzberg PL
Cell reports 2021 Oct 12;37(2):109804

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LAL using 30 µg of A) A549 and B) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a LAL polyclonal antibody (Product # PA5-27346) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using LIPA Polyclonal Antibody (Product # PA5-27346). Sample (50 µg of whole cell lysate). Lane A: mouse Liver. 10% SDS PAGE. LIPA Polyclonal Antibody (Product # PA5-27346) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using LIPA Polyclonal Antibody (Product # PA5-27346). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIPA Polyclonal Antibody (Product # PA5-27346) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LIPA was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a LIPA Polyclonal Antibody (Product # PA5-27346) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LIPA Polyclonal Antibody detects LAL protein at lysosome by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: LAL stained by LIPA Polyclonal Antibody (Product # PA5-27346) diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LIPA Polyclonal Antibody detects LAL protein at lysosome by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: LAL stained by LIPA Polyclonal Antibody (Product # PA5-27346) diluted at 1:500. Blue: Fluoroshield with DAPI .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. IL-15 differentiated Pik3cd E1020K/+ OT-1 CD8 + T cells resemble effector cells (A-E) OT-1 cells activated with OVA 257-264 for 3 days and then cultured with either IL-2 or IL-15 to generate effector-like or memory-like cells, respectively (see Figure S6A ). (A) GzmB, CD69, and CD62L (n=3, representative flow plots). (B-E) RNA-seq analysis. (B) Principal-component analysis (PCA) of the two most variant TPM data components (WT cells, circles; Pik3cd E1020K/+ , triangles). (C) Heatmap of TPM values and Euclidian clustering for DEGs (rows) and experimental groups (columns). (D and E) Enrichment across 10 row clusters (C) of KEGG pathways (D) or curated CD8 + T cell gene sets (E) from http://www.gsea-msigdb.org/gsea/index.jsp . (F and G) OCR under basal conditions and in response to mitochondrial inhibitors oligomycin, FCCP, and antimycin A plus rotenone and calculated as the percent SRC in cells differentiated in IL-2 (F) and IL-15 (G) (representative data, n = 4). (H) LAL, Tip47, and AKT from IL-2 or IL-15 cultured cells (n = 2). Graphs (F and G) show mean +- SEM, percent SRC p < 0.0001. See Figure S7 and Table S2 .