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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00029916-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00029916-M05, RRID:AB_1137045
- Product name
- SNX11 monoclonal antibody (M05), clone 2G1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant SNX11.
- Antigen sequence
MGFWCRMSENQEQEEVITVRVQDPRVQNEGSWNSY
VDYKIFLHTNSKAFTAKTSCVRRRYREFVWLRKQL
QRNAGLVPVPELPGKSTFFGTSDEFIEKRRQGLQH
FLEKVLQSVVLLSDSQLHLFLQSQLSVPEIEACVQ
GRSTMTVSDAILRYAMSNCGWAQEERQSSSHLAKG
DQPKSCCFLPRSGRRSSPSPPPSEEKDHLEVWAPV
VDSEVPSLESPTLPPLSSPLCCDFGRPKEGTSTLQ
SVRRAVGGDHAVPLDPGQLETVLEK- Isotype
- IgG
- Antibody clone number
- 2G1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SNX11 expression in transfected 293T cell line by SNX11 monoclonal antibody (M05), clone 2G1.Lane 1: SNX11 transfected lysate(30.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SNX11 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SNX11 transfected lysate using anti-SNX11 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SNX11 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
