PA5-93101

antibody from Invitrogen Antibodies

Targeting: HOXB1 HOX2, HOX2I

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93101

Provider

Invitrogen Antibodies

Product name

HOXB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SAQAVDSYAS EGRYGGGLSS PAFQQNSGYP AQQPPSTLGV PFPSSAPSGY APAACSPSYG PSQYYPLGQS EGDGGYFHPS SYGAQLGGLS DGYGAGGAGP GPYPPQHPPY GNEQTASFAP AYADLLSEDK ETPCPSEPNT PTARTF; Positive Samples: Mouse heart; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.12 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of mouse heart, using HOXB1 Polyclonal antibody (Product # PA5-93101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HOXB1 in extracts of mouse heart using HOXB1 Polyclonal Antibody (Product # PA5-93101) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HOXB1 was performed in U-2 OS cells using HOXB1 Polyclonal Antibody (Product # PA5-93101) at a dilution of 1:100. Blue: DAPI for nuclear staining.