TA500726

antibody from OriGene

Targeting: IFIT3 CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, RIG-G

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500726

Provider

OriGene

Proper citation

OriGene Cat#TA500726, RRID:AB_11127767

Product name

Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Antibody type

Monoclonal

Description

Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Host

Mouse

Conjugate

Unconjugated

Epitope

IFIT3

Isotype

IgG

Antibody clone number

OTI1G1

Vial size

100 µl

Concentration

NULL

References

Submitted references

Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability.

Johnson B, VanBlargan LA, Xu W, White JP, Shan C, Shi PY, Zhang R, Adhikari J, Gross ML, Leung DW, Diamond MS, Amarasinghe GK
Immunity 2018 Mar 20;48(3):487-499.e5

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (RC200610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-IFIT3 mouse monoclonal antibody (TA500726) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3(RC200610).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody (TA500726).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-IFIT3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500726, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-IFIT3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500726, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-IFIT3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500726, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-IFIT3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500726, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-IFIT3 antibody(TA500726),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

Validation comment

FC