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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX132002 - Provider product page
- Provider
- GeneTex
- Product name
- Pyruvate Carboxylase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody (GTX132002) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCB antibody detects PCB protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.Green: PCB protein stained by PCB antibody (GTX132002) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCB protein from HeLa whole cell extracts using 5 £gg of PCB antibody (GTX132002).Western blot analysis was performed using PCB antibody (GTX132002).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCB antibody detects PCB protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. PCB antibody (GTX132002) diluted at 1:500.
