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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-50101 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pyruvate Carboxylase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total Pyruvate Carboxylase protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.
Kerr AG, Wang Z, Wang N, Kwok KHM, Jalkanen J, Ludzki A, Lecoutre S, Langin D, Bergo MO, Dahlman I, Mim C, Arner P, Gao H
Nature communications 2022 May 26;13(1):2958
Nature communications 2022 May 26;13(1):2958
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), 3T3-L1 (Lane 3), 3T3-L1 differentiated to adipocytes (Lane 4), tissue extracts of Mouse Liver (Lane 5) and Mouse Brown Adipose (Lane 6). The blot was probed with Anti-Pyruvate Carboxylase Polyclonal Antibody (Product # PA5-50101, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 130 kDa band corresponding to Pyruvate Carboxylase was observed across cell lines, tissues tested and was enhanced in 3T3-L1 upon differentiation to adipocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Pyruvate carboxylase was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Pyruvate Carboxylase Polyclonal Antibody (Product # PA5-50101) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PC in paraffin-embedded human lung carcinoma tissue. Samples were probed using a PC polyclonal antibody (Product # PA5-50101).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Pyruvate Carboxylase in paraffin-embedded Human lung carcinoma tissue using Pyruvate Carboxylase Polyclonal Antibody (Product # PA5-50101).
