16588-1-AP

antibody from Invitrogen Antibodies

Targeting: PC PCB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16588-1-AP

Provider

Invitrogen Antibodies

Product name

Pyruvate Carboxylase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MERVFDYSE YWEGARGLYA AFDCTATMKS GNSDVYENEI PGGQYTNLHF QAHSMGLGSK FKEVKKAYVE ANQMLGDLIK VTPSSKIVGD LAQFMVQNGL SRAEAEAQAE ELSFPRSVVE FLQGYIGVPH GGFPEPFRSK VLKDLPRVEG RPGASLPPLD LQALEKELVD RHGEEVTPED VLSAAMYPDV FAHFKDFTAT FGPLDSLNTR LFLQGPKIAE EFEVELERGK TLHIKALAVS DLNRAGQRQV FFELNGQLRS ILVKDTQAMK EMHFHPKALK DVKGQIGAPM PGKVIDIKVV AGAKVAKGQP LCVLSAMKME TVVTSPMEGT VRKVHVTKDM TLEGDDLILE IE (828-1178 aa encoded by BC011617)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PC antibody 16588-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 16588-1-AP) (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.