PA5-88766

antibody from Invitrogen Antibodies

Targeting: GPC4 K-glypican

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88766

Provider

Invitrogen Antibodies

Product name

Glypican 4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SESAFSARFR PHHPEERPTT AAGTSLDRLV TDVKEKLKQA KKFWSSLPSN VCNDERMAAG NGNEDDCWNG KGKSRYLFAV TGNGLANQGN NPEVQVDTSK PDILILRQIM ALRVMTSKMK NAYNGNDVDF FDISDESSGE GSGSGCEYQQ CPSEFDYNAT DHAGKSANEK ADSAGVRPGA QAYLLTVFCI LFLVMQREWR; Positive Samples: U-87MG, A-431, 293T; Cellular Location: Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, extracellular space

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.62 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using GPC4 Polyclonal antibody (Product # PA5-88766) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Glypican 4 in extracts of various cell lines using Glypican 4 Polyclonal Antibody (Product # PA5-88766) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Glypican 4 in extracts of various cell lines using Glypican 4 Polyclonal Antibody (Product # PA5-88766) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using GPC4 Polyclonal antibody (Product # PA5-88766) at dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using GPC4 Polyclonal antibody (Product # PA5-88766) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Glypican 4 in paraffin-embedded human liver using Glypican 4 Polyclonal Antibody (Product # PA5-88766) at a dilution of 1:100.