21324-1-AP

antibody from Invitrogen Antibodies

Targeting: ZDHHC5 DHHC5, KIAA1748, ZNF375

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21324-1-AP

Provider

Invitrogen Antibodies

Product name

ZDHHC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AMPHSSSAK LSRGDSLKEP TSIAESSRHP SYRSEPSLEP ESFRSPTFGK SFHFDPLSSG SRSSSLKSAQ GTGFELGQLQ SIRSEGTTST SYKSLANQTR NGSLSYDSLL TPSDSPDFES VQAGPEPDPP LGYTSPFLSA RLAQQREAER HPRLVPTGPT HREPSPVRYD NLSRHIVASL QEREKLLRQS PPLPGREEEP GLGDSGIQST PGSGHAPRTS SSSDDSKRSP LGKTPLGRPA VPRFGKPDGL RGRGVGSPEP GPTAPYLGRS MSYSSQKAQP GVSETEEVAL QPLLTPKDEV QLKTTYSKSN GQPKSLGSAS PGPGQPPLSS PTRGGVKKVS GVGGTTYEIS V (366-715 aa encoded by BC026967)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug ZDHHC5 antibody (21324-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.