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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AM31112PU-N - Provider product page
- Provider
- OriGene
- Product name
- SMURF1 (165-269) mouse monoclonal antibody, clone 1D7, Purified
- Antibody type
- Monoclonal
- Description
- SMURF1 (165-269) mouse monoclonal antibody, clone 1D7, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SMURF1
- Isotype
- IgG
- Antibody clone number
- 1D7
- Vial size
- 50 µg
- Concentration
- 0.26 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of SMURF1 expression in Raw 264.7 cells with SMURF1 monoclonal antibody, clone 1D7.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody, clone 1D7.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
