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- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ72828 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ZNRF1-Mouse antibody (Internal) (STJ72828)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-ZNRF1-Mouse (Internal) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
SDSTYAHGNGYQET- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with STJ72828 (1µg/ml) , also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72828 (10µg/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
