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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-37982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with human, rat, dog, pig and cow based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of ZNRF1 in N2a cells overexpressing mouse Znrf1 (mock transfection, lane 1) and mouse brain lysates (embryo E14 and adult cerebellum, lane 2). Lysates were probed with a ZNRF1 polyclonal antibody (Product # PA5-37982) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ZNRF1 in paraffin embedded mouse cerebral cortex using a ZNRF1 polyclonal antibody (Product #PA5-37982) at a concentration of 1:100. Microwaved antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with streptavidfine-Alexa 488 after biotinylation with an anti-goat secondary antibody. The Neurofilament M was labeled with a different antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZNRF1 in paraffin-embedded mouse cerebral cortex tissue following microwave antigen retrieval with a citrate buffer, pH 6, using a ZNRF1 polyclonal antibody (Product # PA5-37982) at a dilution of 10 µg/mL. Detection of ZNRF1 was performed using a biotinylated anti-goat secondary antibody followed by streptavidin-Alexa Fluor 488 (green, left). Neurofilament M was stained using a labeled secondary antibody at a dilution of 1:100 (red, center), and a merged image is shown on the right.
