MA5-27809

antibody from Invitrogen Antibodies

Targeting: CFDP1 BCNT, CENP-29, CP27, p97, SWC5, Yeti

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27809

Provider

Invitrogen Antibodies

Product name

CFDP1 Monoclonal Antibody (GT11811)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS

Antibody clone number

GT11811

Concentration

2.34 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CFDP1 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with CFDP1 monoclonal antibody (Product # MA5-27809) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CFDP1 in A) U87-MG whole cell lysate, B) SK-N-SH whole cell lysate, C) IMR32 whole cell lysate, D) SK-N-AS whole cell lysate using 20 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with CFDP1 monoclonal antibody (Product # MA5-27809) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CFDP1 Monoclonal Antibody (GT11811) detects CFDP1 protein by western blot analysis. A U87-MG whole cell lysate/extract. B SK-N-SH whole cell lysate/extract. C IMR32 whole cell lysate/extract. D SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CFDP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) diluted at 1:100. Red: alpha Tubulin 4a, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human ovarian cancer tissue using CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of CFDP1 was performed in paraffin-embedded human breast carcinoma tissue using CFDP1 Monoclonal Antibody (GT11811) (Product # MA5-27809) at a dilution of 1:2000.