Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47740 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHST5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHST5 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in CHST5 polyclonal antibody (Product # PA5-47740) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.