Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-56966 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCAN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LRDTMKSWND SQSDLCSTDQ EEEEEMIFGE NEDDLDEMMD LSDLPTSLFA CSVHEAVFEA REQKERFEAL FTIYDD Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RCAN3 in control (vector only transfected HEK293T lysate) and RCAN3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RCAN3 Polyclonal Antibody (Product # PA5-56966).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RCAN3 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a RCAN3 Polyclonal Antibody (Product # PA5-56966).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RCAN3 in human prostate and endometrium tissues using RCAN3 Polyclonal Antibody (Product # PA5-56966). Corresponding RCAN3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RCAN3 in human endometrium using RCAN3 Polyclonal Antibody (Product # PA5-56966) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RCAN3 in human prostate using RCAN3 Polyclonal Antibody (Product # PA5-56966) shows high expression.