A-100

antibody from R&D Systems

Targeting: RPS27A S27A, Uba80, UBCEP80

Western blot (Data presented on provider website)

Antibody Data

Product number

A-100

Provider

R&D Systems

Product name

Human Ubiquitin Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. The antibody cross-reacts with mouse, rat and human ubiquitin; but not with Nedd8 or Sumo1 proteins. This reagent is non-functional for immunoprecipitation.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

200 uL

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

References

Submitted references

UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells.

Kim HT, Goldberg AL
Proceedings of the National Academy of Sciences of the United States of America 2018 Dec 11;115(50):E11642-E11650

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RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor β signaling.

Malonis RJ, Fu W, Jelcic MJ, Thompson M, Canter BS, Tsikitis M, Esteva FJ, Sánchez I
The Journal of biological chemistry 2017 May 5;292(18):7435-7451

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The deubiquitinating enzyme Usp14 allosterically inhibits multiple proteasomal activities and ubiquitin-independent proteolysis.

Kim HT, Goldberg AL
The Journal of biological chemistry 2017 Jun 9;292(23):9830-9839

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Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation.

Perea-Resa C, Rodríguez-Milla MA, Iniesto E, Rubio V, Salinas J
Molecular plant 2017 Jun 5;10(6):791-804

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Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.

Pereira MG, Baptista IL, Carlassara EO, Moriscot AS, Aoki MS, Miyabara EH
PloS one 2014;9(1):e85283