PAB20729

antibody from Abnova Corporation

Targeting: TMEM143 FLJ10922

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20729

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20729, RRID:AB_10966946

Product name

TMEM143 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TMEM143.

Antigen sequence

MWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFH
SSPAEKAALEAFSAHVDFCTLFHYHQILARLQALY
DPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLL
AQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQY
V


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TMEM143 polyclonal antibody (Cat # PAB20729) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with TMEM143 polyclonal antibody (Cat # PAB20729) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)