A304-153A

antibody from Invitrogen Antibodies

Targeting: GAS2L3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A304-153A

Provider

Invitrogen Antibodies

Product name

GAS2L3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GAS2L3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, K562, MCF7, and Hep G2 cells. Antibodies: Affinity purified rabbit anti-GAS2L3 antibody A304-153A (lot A304-153A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GAS2L3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GAS2L3 antibody A304-153A (lot A304-153A-1) used for IP at 6 µg/mg lysate. GAS2L3 was also immunoprecipitated by rabbit anti-GAS2L3 antibody A304-152A. For blotting immunoprecipitated GAS2L3, A304-153A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GAS2L3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, K562, MCF7, and Hep G2 cells. Antibodies: Affinity purified rabbit anti-GAS2L3 antibody A304-153A (lot A304-153A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.