MA5-34821

antibody from Invitrogen Antibodies

Targeting: IMPDH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34821

Provider

Invitrogen Antibodies

Product name

IMPDH2 Recombinant Rabbit Monoclonal Antibody (JE40-87)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Daudi, A431, LOVO, PC-3M, rat kidney tissue, human tonsil tissue, human thyroid gland tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE40-87

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IMPDH2 in Daudi cell lysate. Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), at a dilution of 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IMPDH2 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IMPDH2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IMPDH2 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IMPDH2 in paraffin-embedded rat kidney tissue. Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IMPDH2 in paraffin-embedded mouse testis tissue. Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IMPDH2 in paraffin-embedded human tonsil tissue. Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IMPDH2 in paraffin-embedded human thyroid gland tissue. Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of IMPDH2 in Daudi cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with IMPDH2 monoclonal antibody (Product # MA5-34821) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.