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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-80897 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHOSPHO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHOSPHO1 in Lane A: Hela Whole Cell Lysate (30 µg). Samples were probed using a PHOSPHO1 Polyclonal Antibody (Product # PA5-80897) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:30 kDa. Observed band size:30 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PHOSPHO1 Polyclonal Antibody (Product # PA5-80897) at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PHOSPHO1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with PHOSPHO1 Polyclonal Antibody (Product # PA5-80897) and 15 µL of 50 % Protein G agarose. Primary antibody: PHOSPHO1 Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.
