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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- PA5-142257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carabin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Carabin in Jurkat (A), (0.3 µg/mL) PBM (B) and negative control A431 (C) cell lysate (35 µg protein in RIPA buffer) using Carabin Polyclonal Antibody (Product # PA5-142257) at 0.5 µg/mL. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Carabin in Human Spleen (A) and Mouse Thymus (B) lysate (35 µg protein in RIPA buffer) using Carabin Polyclonal Antibody (Product # PA5-142257) at 2 µg/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Carabin in Jurkat cells (blue line) using Carabin Polyclonal Antibody (Product # PA5-142257). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
