AB4315

antibody from Novus Biologicals

Targeting: DLL3 SCDO1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AB4315

Provider

Novus Biologicals

Product name

Goat Polyclonal DLL3 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human DLL3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) DLL1 is observed, and less than 8% cross-reactivity with rhDLL4 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human DLL3 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DLL3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of DLL3 in U-118-MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).