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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00005859-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005859-M01, RRID:AB_2173003
- Product name
- Mouse Monoclonal QARS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. QARS - glutaminyl-tRNA synthetase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: QARS Antibody (5F5) [H00005859-M01] - Analysis of QARS expression in transfected 293T cell line by QARS monoclonal antibody (M01), clone 5F5.Lane 1: QARS transfected lysate(87.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: QARS Antibody (5F5) [H00005859-M01] - QARS monoclonal antibody (M01), clone 5F5 Analysis of QARS expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: QARS Antibody (5F5) [H00005859-M01] - Detection limit for recombinant GST tagged QARS is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: QARS Antibody (5F5) [H00005859-M01] - Analysis of QARS transfected lysate using anti-QARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with QARS MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: QARS Antibody (5F5) [H00005859-M01] - Analysis of monoclonal antibody to QARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
