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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RABGAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain.
- Concentration
- 0.83 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RABGAP1 Polyclonal Antibody (Product # PA5-30484). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. RABGAP1 Polyclonal Antibody (Product # PA5-30484) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RABGAP1 Polyclonal Antibody (Product # PA5-30484). Sample (30 µg of whole cell lysate). Lane A: IMR32. 7.5% SDS PAGE. RABGAP1 Polyclonal Antibody (Product # PA5-30484) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RABGAP1 in methanol-fixed HeLa cells using a RABGAP1 polyclonal antibody (Product # PA5-30484) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using RABGAP1 (Product # PA5-30484) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
