Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-75402 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRP7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Microbial metabolite restricts 5-fluorouracil-resistant colonic tumor progression by sensitizing drug transporters via regulation of FOXO3-FOXM1 axis.
Ghosh S, Singh R, Vanwinkle ZM, Guo H, Vemula PK, Goel A, Haribabu B, Jala VR
Theranostics 2022;12(12):5574-5595
Theranostics 2022;12(12):5574-5595
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRP7 in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate. Samples were incubated with MRP7 polyclonal antibody (Product # PA5-75402) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRP7 in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: the Spleen tissue lysate of mouse (40 µg), Lane 4: SGC7901 whole cell lysate (40 µg), Lane 5: H9C2 whole cell lysate (40 µg). Samples were incubated with MRP7 polyclonal antibody (Product # PA5-75402) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Treatment with UroA/UAS03 regulates the FOXO3-FOXM1 axis. A. Total RNA was isolated from parental HCT-116 and HCT-116-FUR cells and analyzed expression of FOXO3 and FOXM1.The fold changes in mRNA levels were determined by RT PCR method. B. Western blot analysis of FOXO3 and FOXM1in parental HCT-116 and HCT-116-FUR cells. C. Total RNA was isolated from HCT-116-FUR cells treated with either 5FU (50 muM) or UroA (50 muM) or UAS03 (50 muM) or in combination for 24 h and analyzed for the expression of FOXO3, FOXM1 and MRP7 by SyBR RT PCR. The fold changes in mRNA levels were represented. D. HCT-116-FUR cells were treated with either 5FU (50 muM) or UroA (50 muM) or UAS03 (50 muM) or in combination for 24 h. Western blot analysis of FOXO3, FOXM1 and MRP7. Statistics performed one-way ANOVA using Graph Prism Software. Error bars, +-SEM. **p < 0.01, ***p < 0.001.