Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ANX-006-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Na+/H+ Exchanger 6 (NHE-6) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200). 2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody , preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200). 2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody , preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4): - 1,2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).3,4. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody , preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysate: - 1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200). 2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody , preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NHE-6 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Na+/H+Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. NHE-6 staining (green) in hippocampal dentate gyrus region, appears in neuronal soma outlines in the hilus (horizontal arrows) and the outer granule layer (vertical arrows). Cell nuclei were stained with DAPI (blue).