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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX118737 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118737, RRID:AB_10617865
- Product name
- PNPase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Helicase SUV3, polynucleotide phosphorylase, and mitochondrial polyadenylation polymerase form a transient complex to modulate mitochondrial mRNA polyadenylated tail lengths in response to energetic changes.
Wang DD, Guo XE, Modrek AS, Chen CF, Chen PL, Lee WH
The Journal of biological chemistry 2014 Jun 13;289(24):16727-35
The Journal of biological chemistry 2014 Jun 13;289(24):16727-35
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX118737 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PNPase antibody detects PNPase protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody (GTX118737) diluted by 1:2500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase(GTX118737) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PNPase protein from HeLa whole cell extracts using 5 £gg of PNPase antibody (GTX118737).Western blot analysis was performed using PNPase antibody (GTX118737).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. PNPase antibody (GTX118737) dilution: 1:250.
