H00087178-D01

antibody from Abnova Corporation

Targeting: PNPT1 DFNB70, old-35, OLD35, PNPase

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00087178-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00087178-D01, RRID:AB_10718524

Product name

PNPT1 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PNPT1 protein.

Antigen sequence

MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQV
RALWSSAGSRAVAVDLGNRKLEISSGKLARFADGS
AVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQK
AAAAGRIPTNYLRREVGTSDKEILTSRIIDRSIRP
LFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASV
ALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMS
SSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAI
KVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPS
PEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNK
IRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRS
IVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSAL
FQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKD
KNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAE
KALYPVIPRDFPFTIRVTSEVLESNGSSSMASACG
GSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIE
DYRLLTDILGIEDYNGDMDFKIAGTNKGITALQAD
IKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISK
PRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKK
LQAETGVTISQVDEETFSVFAPTPSAMHEARDFIT
EICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYP
NMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKY
FGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIV
MGEPISQSSSNSQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PNPT1 expression in transfected 293T cell line (H00087178-T01) by PNPT1 MaxPab polyclonal antibody.Lane 1: PNPT1 transfected lysate(85.90 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of PNPT1 transfected lysate using anti-PNPT1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PNPT1 purified MaxPab mouse polyclonal antibody (B01P) (H00087178-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol