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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA1-16766 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: mouse testis protein.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Feline, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Characterization of Estrogenic Activity and Site-Specific Accumulation of Bisphenol-A in Epididymal Fat Pad: Interfering Effects on the Endocannabinoid System and Temporal Progression of Germ Cells.
Chioccarelli T, Migliaccio M, Suglia A, Manfrevola F, Porreca V, Diano N, Errico S, Fasano S, Cobellis G
International journal of molecular sciences 2021 Mar 3;22(5)
International journal of molecular sciences 2021 Mar 3;22(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCP3 in mouse testis protein using Product # PA1-16766.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCP3 in mouse testis protein. Sample was incubated in SCP3 polyclonal antibody (Product # PA1-16766).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SCP3 in mouse pachytene preparation (red). Samples were incubated in SCP3 polyclonal antibody (Product # PA1-16766). CDK2 staining, near teleomeres, is also present (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SCP3 in formalin fixed and paraffin embedded tissue section of Mouse testis. Samples were incubated in SCP3 polyclonal antibody (Product # PA1-16766) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SCP3 in formalin fixed paraffin embedded tissue section of mouse testes. Samples were incubated in SCP3 polyclonal antibody (Product # PA1-16766) using a dilution of 1:200. Specific staining may be seen in the spermatogonial cells and the primary spermatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SCP3 in human testis. Samples were incubated in SCP3 polyclonal antibody (Product # PA1-16766) followed by DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Gene expression analysis of germ cells markers in testis of male mice early-life exposed to vehicle (CTRL, n = 3/4) or BPA (BPA, n = 4) and sacrificed at 78 dpp . Western Blot analysis of VASA, MAGE-A4, SCP3 and TNP2. Protein amounts were quantified by densitometry analysis, normalized against ERK-2 signals, and expressed in OD values as fold change ( A - D ). All data were reported as mean value +- S.E.M. (*: p < 0.05; **: p < 0.01).
