Antibody data
- Antibody Data
- Antigen structure
- References [48]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
Reference
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- Product number
- NB300-232 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-232, RRID:AB_2087193
- Product name
- Rabbit Polyclonal SCP3/SYCP3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Feline, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
Oestrogen in the chick embryo can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex that can support oogenesis.
UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I.
BRUCE preserves genomic stability in the male germline of mice.
Dysregulation of FGFR signalling by a selective inhibitor reduces germ cell survival in human fetal gonads of both sexes and alters the somatic niche in fetal testes.
Chemical Contaminants from Plastics in the Animal Environment.
The testis-specifically expressed Dpep3 is not essential for male fertility in mice.
Meiotic Synapsis and Gene Expression Altered by a Balanced Y-Autosome Reciprocal Translocation in an Azoospermic Pig.
Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis.
Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice.
Nicotine exposure impairs germ cell development in human fetal ovaries cultured in vitro.
Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring.
Effect of low-dose zearalenone exposure on reproductive capacity of male mice.
In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells.
XACT Noncoding RNA Competes with XIST in the Control of X Chromosome Activity during Human Early Development.
Characterization of the testicular regeneration potential in premature cockerels.
A homozygous FANCM mutation underlies a familial case of non-syndromic primary ovarian insufficiency.
Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro.
The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis.
Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis.
Fasciola hepatica: a light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy.
Overexpression of Anti-Müllerian Hormone Disrupts Gonadal Sex Differentiation, Blocks Sex Hormone Synthesis, and Supports Cell Autonomous Sex Development in the Chicken.
On the development of extragonadal and gonadal human germ cells.
Polycomb repressive complex 1 (PRC1) regulates meiotic initiation of ovarian germ cells in chick embryos.
Mouse Tafazzin Is Required for Male Germ Cell Meiosis and Spermatogenesis.
Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration.
Meiotic wave adds extra asymmetry to the development of female chicken gonads.
Activin A accelerates the progression of fetal oocytes throughout meiosis and early oogenesis in the mouse.
The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse.
Cytological studies of human meiosis: sex-specific differences in recombination originate at, or prior to, establishment of double-strand breaks.
Regulation of the DNA damage response on male meiotic sex chromosomes.
Germline mosaicism does not explain the maternal age effect on trisomy.
No evidence for neo-oogenesis may link to ovarian senescence in adult monkey.
Dysregulation of the mitosis-meiosis switch in testicular carcinoma in situ.
Deleted in azoospermia-like enhances in vitro derived porcine germ cell formation and meiosis.
The ontogeny of cKIT+ human primordial germ cells proves to be a resource for human germ line reprogramming, imprint erasure and in vitro differentiation.
Recovery of functional oocytes from cultured premeiotic germ cells after kidney capsule transplantation.
In vitro production of haploid cells after coculture of CD49f+ with Sertoli cells from testicular sperm extraction in nonobstructive azoospermic patients.
Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus).
Divergent RNA-binding proteins, DAZL and VASA, induce meiotic progression in human germ cells derived in vitro.
HORMAD1-dependent checkpoint/surveillance mechanism eliminates asynaptic oocytes.
Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women.
A novel mouse synaptonemal complex protein is essential for loading of central element proteins, recombination, and fertility.
Analyzing mammalian female meiosis.
Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis.
Small ubiquitin-related modifier (SUMO)-1, SUMO-2/3 and SUMOylation are involved with centromeric heterochromatin of chromosomes 9 and 1 and proteins of the synaptonemal complex during meiosis in men.
Germline stem cells and neo-oogenesis in the adult human ovary.
Abe H, Alavattam KG, Hu YC, Pang Q, Andreassen PR, Hegde RS, Namekawa SH
Current biology : CB 2020 Feb 3;30(3):408-420.e5
Current biology : CB 2020 Feb 3;30(3):408-420.e5
Oestrogen in the chick embryo can induce chromosomally male ZZ left gonad epithelial cells to form an ovarian cortex that can support oogenesis.
Guioli S, Zhao D, Nandi S, Clinton M, Lovell-Badge R
Development (Cambridge, England) 2020 Feb 25;147(4)
Development (Cambridge, England) 2020 Feb 25;147(4)
UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I.
Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, Gao Q, Li L, Wu H, Zheng H, Qi Q, Li T, Zhang M, Zhang L, Wan X, Lin X, Wong J, Shi Q, Li R
Cell death & disease 2020 Feb 21;11(2):142
Cell death & disease 2020 Feb 21;11(2):142
BRUCE preserves genomic stability in the male germline of mice.
Che L, Alavattam KG, Stambrook PJ, Namekawa SH, Du C
Cell death and differentiation 2020 Aug;27(8):2402-2416
Cell death and differentiation 2020 Aug;27(8):2402-2416
Dysregulation of FGFR signalling by a selective inhibitor reduces germ cell survival in human fetal gonads of both sexes and alters the somatic niche in fetal testes.
Harpelunde Poulsen K, Nielsen JE, Frederiksen H, Melau C, Juul Hare K, Langhoff Thuesen L, Perlman S, Lundvall L, Mitchell RT, Juul A, Rajpert-De Meyts E, Jørgensen A
Human reproduction (Oxford, England) 2019 Nov 1;34(11):2228-2243
Human reproduction (Oxford, England) 2019 Nov 1;34(11):2228-2243
Chemical Contaminants from Plastics in the Animal Environment.
Gorence GJ, Pulcastro HC, Lawson CA, Gerona RR, Friesen M, Horan TS, Gieske MC, Sartain CV, Hunt PA
Journal of the American Association for Laboratory Animal Science : JAALAS 2019 Mar 1;58(2):190-196
Journal of the American Association for Laboratory Animal Science : JAALAS 2019 Mar 1;58(2):190-196
The testis-specifically expressed Dpep3 is not essential for male fertility in mice.
Xie Y, Khan R, Wahab F, Hussain HMJ, Ali A, Ma H, Jiang H, Xu J, Zaman Q, Khan M, Jiang X, Shi Q
Gene 2019 Aug 30;711:143925
Gene 2019 Aug 30;711:143925
Meiotic Synapsis and Gene Expression Altered by a Balanced Y-Autosome Reciprocal Translocation in an Azoospermic Pig.
Mary N, Villagómez DAF, Revay T, Rezaei S, Donaldson B, Pinton A, King WA
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 2018 Sep 5;12(5)
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 2018 Sep 5;12(5)
Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis.
Jørgensen A, Macdonald J, Nielsen JE, Kilcoyne KR, Perlman S, Lundvall L, Langhoff Thuesen L, Juul Hare K, Frederiksen H, Andersson AM, Skakkebæk NE, Juul A, Sharpe RM, Rajpert-De Meyts E, Mitchell RT
Cell reports 2018 Nov 13;25(7):1924-1937.e4
Cell reports 2018 Nov 13;25(7):1924-1937.e4
Retinoic acid-induced CYP51 nuclear translocation promotes meiosis prophase I process and is correlated to the expression of REC8 and STAG3 in mice.
Mu X, Wen J, Chen Q, Wang Z, Wang Y, Guo M, Yang Y, Xu J, Wei Z, Xia G, Yang M, Wang C
Biology open 2018 Nov 12;7(11)
Biology open 2018 Nov 12;7(11)
Nicotine exposure impairs germ cell development in human fetal ovaries cultured in vitro.
Cheng SF, Qin XS, Han ZL, Sun XF, Feng YN, Yang F, Ge W, Li L, Zhao Y, De Felici M, Zou SH, Zhou Y, Shen W
Aging 2018 Jul 12;10(7):1556-1574
Aging 2018 Jul 12;10(7):1556-1574
Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring.
Zhai QY, Ge W, Wang JJ, Sun XF, Ma JM, Liu JC, Zhao Y, Feng YZ, Dyce PW, De Felici M, Shen W
Aging 2018 Aug 28;10(8):2170-2189
Aging 2018 Aug 28;10(8):2170-2189
Effect of low-dose zearalenone exposure on reproductive capacity of male mice.
Pang J, Zhou Q, Sun X, Li L, Zhou B, Zeng F, Zhao Y, Shen W, Sun Z
Toxicology and applied pharmacology 2017 Oct 15;333:60-67
Toxicology and applied pharmacology 2017 Oct 15;333:60-67
In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells.
Jung D, Xiong J, Ye M, Qin X, Li L, Cheng S, Luo M, Peng J, Dong J, Tang F, Shen W, Matzuk MM, Kee K
Nature communications 2017 Jun 12;8:15680
Nature communications 2017 Jun 12;8:15680
XACT Noncoding RNA Competes with XIST in the Control of X Chromosome Activity during Human Early Development.
Vallot C, Patrat C, Collier AJ, Huret C, Casanova M, Liyakat Ali TM, Tosolini M, Frydman N, Heard E, Rugg-Gunn PJ, Rougeulle C
Cell stem cell 2017 Jan 5;20(1):102-111
Cell stem cell 2017 Jan 5;20(1):102-111
Characterization of the testicular regeneration potential in premature cockerels.
Cheng PL, Wu HR, Li CY, Chen CF, Cheng HC
The Journal of reproduction and development 2017 Dec 15;63(6):563-570
The Journal of reproduction and development 2017 Dec 15;63(6):563-570
A homozygous FANCM mutation underlies a familial case of non-syndromic primary ovarian insufficiency.
Fouquet B, Pawlikowska P, Caburet S, Guigon C, Mäkinen M, Tanner L, Hietala M, Urbanska K, Bellutti L, Legois B, Bessieres B, Gougeon A, Benachi A, Livera G, Rosselli F, Veitia RA, Misrahi M
eLife 2017 Dec 12;6
eLife 2017 Dec 12;6
Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro.
Liu JC, Lai FN, Li L, Sun XF, Cheng SF, Ge W, Wang YF, Li L, Zhang XF, De Felici M, Dyce PW, Shen W
Cell death & disease 2017 Aug 3;8(8):e2966
Cell death & disease 2017 Aug 3;8(8):e2966
The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis.
Liu KH, Sun XF, Feng YZ, Cheng SF, Li B, Li YP, Shen W, Li L
Toxicology and applied pharmacology 2017 Aug 15;329:9-17
Toxicology and applied pharmacology 2017 Aug 15;329:9-17
Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis.
Zhuang XJ, Tang WH, Feng X, Liu CY, Zhu JL, Yan J, Liu DF, Liu P, Qiao J
Cell cycle (Georgetown, Tex.) 2016 Oct;15(19):2576-2584
Cell cycle (Georgetown, Tex.) 2016 Oct;15(19):2576-2584
Fasciola hepatica: a light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy.
Hanna RE, Moffett D, Forster FI, Trudgett AG, Brennan GP, Fairweather I
Veterinary parasitology 2016 May 15;221:93-103
Veterinary parasitology 2016 May 15;221:93-103
Overexpression of Anti-Müllerian Hormone Disrupts Gonadal Sex Differentiation, Blocks Sex Hormone Synthesis, and Supports Cell Autonomous Sex Development in the Chicken.
Lambeth LS, Morris K, Ayers KL, Wise TG, O'Neil T, Wilson S, Cao Y, Sinclair AH, Cutting AD, Doran TJ, Smith CA
Endocrinology 2016 Mar;157(3):1258-75
Endocrinology 2016 Mar;157(3):1258-75
On the development of extragonadal and gonadal human germ cells.
Heeren AM, He N, de Souza AF, Goercharn-Ramlal A, van Iperen L, Roost MS, Gomes Fernandes MM, van der Westerlaken LA, Chuva de Sousa Lopes SM
Biology open 2016 Feb 1;5(2):185-94
Biology open 2016 Feb 1;5(2):185-94
Polycomb repressive complex 1 (PRC1) regulates meiotic initiation of ovarian germ cells in chick embryos.
Tang X, Xu S, Li R, Zhang H, Chen Q, Wu W, Liu H
Molecular and cellular endocrinology 2016 Dec 5;437:171-182
Molecular and cellular endocrinology 2016 Dec 5;437:171-182
Mouse Tafazzin Is Required for Male Germ Cell Meiosis and Spermatogenesis.
Cadalbert LC, Ghaffar FN, Stevenson D, Bryson S, Vaz FM, Gottlieb E, Strathdee D
PloS one 2015;10(6):e0131066
PloS one 2015;10(6):e0131066
Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration.
Ge W, Ma HG, Cheng SF, Sun YC, Sun LL, Sun XF, Li L, Dyce P, Li J, Shi QH, Shen W
Scientific reports 2015 Sep 8;5:13822
Scientific reports 2015 Sep 8;5:13822
Meiotic wave adds extra asymmetry to the development of female chicken gonads.
de Melo Bernardo A, Heeren AM, van Iperen L, Fernandes MG, He N, Anjie S, Noce T, Ramos ES, de Sousa Lopes SM
Molecular reproduction and development 2015 Oct;82(10):774-86
Molecular reproduction and development 2015 Oct;82(10):774-86
Activin A accelerates the progression of fetal oocytes throughout meiosis and early oogenesis in the mouse.
Liang GJ, Zhang XF, Wang JJ, Sun YC, Sun XF, Cheng SF, Li L, De Felici M, Shen W
Stem cells and development 2015 Oct 15;24(20):2455-65
Stem cells and development 2015 Oct 15;24(20):2455-65
The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse.
Link J, Jahn D, Schmitt J, Göb E, Baar J, Ortega S, Benavente R, Alsheimer M
PLoS genetics 2013;9(1):e1003261
PLoS genetics 2013;9(1):e1003261
Cytological studies of human meiosis: sex-specific differences in recombination originate at, or prior to, establishment of double-strand breaks.
Gruhn JR, Rubio C, Broman KW, Hunt PA, Hassold T
PloS one 2013;8(12):e85075
PloS one 2013;8(12):e85075
Regulation of the DNA damage response on male meiotic sex chromosomes.
Lu LY, Xiong Y, Kuang H, Korakavi G, Yu X
Nature communications 2013;4:2105
Nature communications 2013;4:2105
Germline mosaicism does not explain the maternal age effect on trisomy.
Rowsey R, Kashevarova A, Murdoch B, Dickenson C, Woodruff T, Cheng E, Hunt P, Hassold T
American journal of medical genetics. Part A 2013 Oct;161A(10):2495-503
American journal of medical genetics. Part A 2013 Oct;161A(10):2495-503
No evidence for neo-oogenesis may link to ovarian senescence in adult monkey.
Yuan J, Zhang D, Wang L, Liu M, Mao J, Yin Y, Ye X, Liu N, Han J, Gao Y, Cheng T, Keefe DL, Liu L
Stem cells (Dayton, Ohio) 2013 Nov;31(11):2538-50
Stem cells (Dayton, Ohio) 2013 Nov;31(11):2538-50
Dysregulation of the mitosis-meiosis switch in testicular carcinoma in situ.
Jørgensen A, Nielsen JE, Almstrup K, Toft BG, Petersen BL, Rajpert-De Meyts E
The Journal of pathology 2013 Mar;229(4):588-98
The Journal of pathology 2013 Mar;229(4):588-98
Deleted in azoospermia-like enhances in vitro derived porcine germ cell formation and meiosis.
Park BW, Shen W, Linher-Melville K, Li J
Stem cells and development 2013 Mar 15;22(6):939-50
Stem cells and development 2013 Mar 15;22(6):939-50
The ontogeny of cKIT+ human primordial germ cells proves to be a resource for human germ line reprogramming, imprint erasure and in vitro differentiation.
Gkountela S, Li Z, Vincent JJ, Zhang KX, Chen A, Pellegrini M, Clark AT
Nature cell biology 2013 Jan;15(1):113-22
Nature cell biology 2013 Jan;15(1):113-22
Recovery of functional oocytes from cultured premeiotic germ cells after kidney capsule transplantation.
Chen B, Zhang L, Tang J, Feng X, Feng Y, Liang G, Wang L, Feng Y, Li L, De Felici M, Shi Q, Shen W
Stem cells and development 2013 Feb 15;22(4):567-80
Stem cells and development 2013 Feb 15;22(4):567-80
In vitro production of haploid cells after coculture of CD49f+ with Sertoli cells from testicular sperm extraction in nonobstructive azoospermic patients.
Riboldi M, Rubio C, Pellicer A, Gil-Salom M, Simón C
Fertility and sterility 2012 Sep;98(3):580-590.e4
Fertility and sterility 2012 Sep;98(3):580-590.e4
Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Jørgensen A, Nielsen JE, Blomberg Jensen M, Græm N, Rajpert-De Meyts E
Molecular human reproduction 2012 Nov;18(11):523-34
Molecular human reproduction 2012 Nov;18(11):523-34
Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus).
Lin ZY, Imamura M, Sano C, Nakajima R, Suzuki T, Yamadera R, Takehara Y, Okano HJ, Sasaki E, Okano H
Reproduction (Cambridge, England) 2012 May;143(5):597-609
Reproduction (Cambridge, England) 2012 May;143(5):597-609
Divergent RNA-binding proteins, DAZL and VASA, induce meiotic progression in human germ cells derived in vitro.
Medrano JV, Ramathal C, Nguyen HN, Simon C, Reijo Pera RA
Stem cells (Dayton, Ohio) 2012 Mar;30(3):441-51
Stem cells (Dayton, Ohio) 2012 Mar;30(3):441-51
HORMAD1-dependent checkpoint/surveillance mechanism eliminates asynaptic oocytes.
Kogo H, Tsutsumi M, Ohye T, Inagaki H, Abe T, Kurahashi H
Genes to cells : devoted to molecular & cellular mechanisms 2012 Jun;17(6):439-54
Genes to cells : devoted to molecular & cellular mechanisms 2012 Jun;17(6):439-54
Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women.
White YA, Woods DC, Takai Y, Ishihara O, Seki H, Tilly JL
Nature medicine 2012 Feb 26;18(3):413-21
Nature medicine 2012 Feb 26;18(3):413-21
A novel mouse synaptonemal complex protein is essential for loading of central element proteins, recombination, and fertility.
Schramm S, Fraune J, Naumann R, Hernandez-Hernandez A, Höög C, Cooke HJ, Alsheimer M, Benavente R
PLoS genetics 2011 May;7(5):e1002088
PLoS genetics 2011 May;7(5):e1002088
Analyzing mammalian female meiosis.
Susiarjo M, Rubio C, Hunt P
Methods in molecular biology (Clifton, N.J.) 2009;558:339-54
Methods in molecular biology (Clifton, N.J.) 2009;558:339-54
Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis.
Caires KC, de Avila J, McLean DJ
Reproduction (Cambridge, England) 2009 Oct;138(4):667-77
Reproduction (Cambridge, England) 2009 Oct;138(4):667-77
Small ubiquitin-related modifier (SUMO)-1, SUMO-2/3 and SUMOylation are involved with centromeric heterochromatin of chromosomes 9 and 1 and proteins of the synaptonemal complex during meiosis in men.
Brown PW, Hwang K, Schlegel PN, Morris PL
Human reproduction (Oxford, England) 2008 Dec;23(12):2850-7
Human reproduction (Oxford, England) 2008 Dec;23(12):2850-7
Germline stem cells and neo-oogenesis in the adult human ovary.
Liu Y, Wu C, Lyu Q, Yang D, Albertini DF, Keefe DL, Liu L
Developmental biology 2007 Jun 1;306(1):112-20
Developmental biology 2007 Jun 1;306(1):112-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCP3/SYCP3 Antibody [NB300-232] - SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC-P analysis of formalin fixed paraffin embedded tissue section of mouse testes using SCP3 antibody at 1:200 dilution. Specific staining may be seen in the spermatogonial cells and the primary spermatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a specific signal of SCP3 in the spermatogonial cells and the spermatocytes. The signal was strongest in the nuclei of spermatogonial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - Analysis of SCP3 in human testis using DAB with hematoxylin counterstain.