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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-59538 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCSH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TLRTGPALLS VRKFTEKHEW VTTENGIGTV GISNFAQEAL GDVVYCSLPE VGTKLNKQDE FGA Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 89%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCSH in Lane 1: Mouse liver tissue. lysate; Lane 2: Rat liver tissue. lysate. Samples were probed using a GCSH Polyclonal Antibody (Product # PA5-59538).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCSH in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a GCSH Polyclonal Antibody (Product # PA5-59538).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCSH in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a GCSH Polyclonal Antibody (Product # PA5-59538).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GCSH in human cell line U-2 OS using GCSH Polyclonal Antibody (Product # PA5-59538) shows localization to vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCSH in human kidney tissue shows strong granular cytoplasmic positivity in cells in tubules. Samples were probed using a GCSH Polyclonal Antibody (Product # PA5-59538).
