NBP1-47865

antibody from Novus Biologicals

Targeting: NEK6 SID6-1512

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47865

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47865, RRID:AB_10010849

Product name

Mouse Monoclonal NEK6 Antibody

Antibody type

Monoclonal

Description

Affinity purified. This antibody is specific for Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6), transcript variant 2.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.75 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NEK6 Antibody (5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NEK6 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEK6 Antibody (OTI5D7) [NBP1-47865] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NEK6 Antibody (OTI5D7) [NBP1-47865] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK6 antibody, and then analyzed by flow cytometry.