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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-21426 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFV1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (99%), Rat (97%), Zebrafish (87%), Xenopus laevis (90%), Chicken (95%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Early-Life Stress Perturbs Key Cellular Programs in the Developing Mouse Hippocampus.
Wei L, Hao J, Lacher RK, Abbott T, Chung L, Colangelo CM, Kaffman A
Developmental neuroscience 2015;37(6):476-88
Developmental neuroscience 2015;37(6):476-88
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFV1 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NDUFV1 polyclonal antibody (Product # PA5-21426) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFV1 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NDUFV1 polyclonal antibody (Product # PA5-21426) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFV1 Polyclonal Antibody (Product # PA5-21426). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 Polyclonal Antibody (Product # PA5-21426) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using NDUFV1 (Product # PA5-21426) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFV1 Polyclonal Antibody detects NDUFV1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NDUFV1 Polyclonal Antibody (Product # PA5-21426) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFV1 Polyclonal Antibody detects NDUFV1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NDUFV1 Polyclonal Antibody (Product # PA5-21426) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
