Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AM31718PU-N - Provider product page
- Provider
- OriGene
- Product name
- RHOA mouse monoclonal antibody, clone 1B12, Purified
- Antibody type
- Monoclonal
- Description
- RHOA mouse monoclonal antibody, clone 1B12, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RHOA
- Isotype
- IgG
- Antibody clone number
- 1B12
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of RHOA expression in HL-60 cell lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to RHOA on HeLa cell at 10 ug/ml.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC