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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AM31718PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31718PU-N, RRID:AB_10978980
- Product name
- anti RhoA
- Antibody type
- Monoclonal
- Antigen
- RHOA antibody was raised against RHOA (AAH01360, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B12
- Vial size
- 50 µg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of RHOA expression in HL-60 cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to RHOA on HeLa cell at 10 µg/ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
