M09275

antibody from Boster Biological Technology

Targeting: HSBP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M09275

Provider

Boster Biological Technology

Product name

Anti-Heat Shock Protein 27 Monoclonal Antibody

Antibody type

Monoclonal

Description

Anti-Heat Shock Protein 27 Monoclonal Antibody tested in WB, ICC, IF, IHC, reactive to Human, unreactive on rodent

Reactivity

Human

Host

Mouse

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at 4℃ for short term, for longer term at -20℃

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot of tissue or whole cell lysates using mouse mAb to HSP27, M09275, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60kDa band is present in all preparations.

Additional image