- HPA041937 - Atlas Antibodies BLVRB antibody

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Western blot analysis in human cell lines A-549 and U-251MG using Anti-BLVRB antibody. Corresponding BLVRB RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Western blot analysis using Anti-BLVRB antibody HPA041937 (A) shows similar pattern to independent antibody HPA041698 (B).
Antibody #2 product nr: HPA041698
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Immunohistochemical staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody HPA041937 (A) shows similar protein distribution across tissues to independent antibody HPA041698 (B).
Antibody #2 product nr: HPA041698
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human spleen shows storng cytoplasmic positivity in subsets of cells in red and white pulp.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Sample type: HUMAN

HPA041937