MAB6932

antibody from Abnova Corporation

Targeting: GYPA CD235a, GPA, MN, MNS

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6932

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6932, RRID:AB_10677582

Product name

GYPA monoclonal antibody, clone A63-B/C2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native GYPA.

Isotype

IgM

Antibody clone number

A63-B/C2

Storage

Store at 4°C.

References

Submitted references

The expression of human blood group antigens during erythropoiesis in a cell culture system.

Southcott MJ, Tanner MJ, Anstee DJ
Blood 1999 Jun 15;93(12):4425-35

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High-efficiency gene transfer into ex vivo expanded human hematopoietic progenitors and precursor cells by adenovirus vectors.

Frey BM, Hackett NR, Bergelson JM, Finberg R, Crystal RG, Moore MA, Rafii S
Blood 1998 Apr 15;91(8):2781-92

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Characterization of a bipotent erythro-megakaryocytic progenitor in human bone marrow.

Debili N, Coulombel L, Croisille L, Katz A, Guichard J, Breton-Gorius J, Vainchenker W
Blood 1996 Aug 15;88(4):1284-96

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

10⁷ human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932).

Validation comment

Flow Cytometry