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- Antigen structure
- References [3]
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- Product number
- PAB11267 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11267, RRID:AB_1714583
- Product name
- SPANXC polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SPANXC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dynamic structure of the SPANX gene cluster mapped to the prostate cancer susceptibility locus HPCX at Xq27.
The SPANX gene family of cancer/testis-specific antigens: rapid evolution and amplification in African great apes and hominids.
The human SPANX multigene family: genomic organization, alignment and expression in male germ cells and tumor cell lines.
Kouprina N, Pavlicek A, Noskov VN, Solomon G, Otstot J, Isaacs W, Carpten JD, Trent JM, Schleutker J, Barrett JC, Jurka J, Larionov V
Genome research 2005 Nov;15(11):1477-86
Genome research 2005 Nov;15(11):1477-86
The SPANX gene family of cancer/testis-specific antigens: rapid evolution and amplification in African great apes and hominids.
Kouprina N, Mullokandov M, Rogozin IB, Collins NK, Solomon G, Otstot J, Risinger JI, Koonin EV, Barrett JC, Larionov V
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 2;101(9):3077-82
Proceedings of the National Academy of Sciences of the United States of America 2004 Mar 2;101(9):3077-82
The human SPANX multigene family: genomic organization, alignment and expression in male germ cells and tumor cell lines.
Zendman AJ, Zschocke J, van Kraats AA, de Wit NJ, Kurpisz M, Weidle UH, Ruiter DJ, Weiss EH, van Muijen GN
Gene 2003 May 8;309(2):125-33
Gene 2003 May 8;309(2):125-33
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using SPANXC polyclonal antibody (Cat # PAB11267) shows detection of a band at ~17 KDacorresponding to SPANXC present in a nuclear extract from VWM105 cells (left panel, arrowhead).VWM105 cells arederived from a human melanoma and arepositive for SPANX proteins.Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein.The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins.Proteins were separatedby SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000.IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left).IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers asindicated.Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
