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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-12916 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal KCNMB3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references KCNMB3 in spinal microglia contributes to the generation and maintenance of neuropathic pain in mice.
BK channels in microglia are required for morphine-induced hyperalgesia.
Imari K, Harada Y, Zhang J, Mori Y, Hayashi Y
International journal of molecular medicine 2019 Oct;44(4):1585-1593
International journal of molecular medicine 2019 Oct;44(4):1585-1593
BK channels in microglia are required for morphine-induced hyperalgesia.
Hayashi Y, Morinaga S, Zhang J, Satoh Y, Meredith AL, Nakata T, Wu Z, Kohsaka S, Inoue K, Nakanishi H
Nature communications 2016 May 31;7:11697
Nature communications 2016 May 31;7:11697
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCNMB3 Antibody (S40B-18) [NBP2-12916] - analysis of Rat brain membrane lysate showing detection of KCNMB3 Potassium Channel protein using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCNMB3 Antibody (S40B-18) [NBP2-12916] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful filaggrinlike staining.
