PAB22331

antibody from Abnova Corporation

Targeting: APEH D3F15S2, D3S48E, DNF15S2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22331

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22331, RRID:AB_10967378

Product name

APEH polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant APEH.

Antigen sequence

RQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPS
GTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFN
LSALEKHGPVYEDDCFGC


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with APEH polyclonal antibody (Cat # PAB22331) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with APEH polyclonal antibody (Cat # PAB22331) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with APEH polyclonal antibody (Cat # PAB22331) shows strong cytoplasmic positivity in tubular cells at 1:1000-1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)