10781-1-AP

antibody from Proteintech Group

Targeting: COTL1 CLP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10781-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10781-1-AP, RRID:AB_2084785

Product name

COTL1 antibody

Antibody type

Polyclonal

Description

KD/KO validated COTL1 antibody (Cat. #10781-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Exposure to cigarette smoke extract and lipopolysaccharide modifies cytoskeleton organization in bronchial epithelial cells.

D'Anna C, Cigna D, Di Sano C, Di Vincenzo S, Dino P, Ferraro M, Bini L, Bianchi L, Di Gaudio F, Gjomarkaj M, Pace E
Experimental lung research 2017 Nov - Dec;43(9-10):347-358

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Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Guo S, Yang P, Jiang X, Li X, Wang Y, Zhang X, Sun B, Zhang Y, Jia Y
Oncotarget 2017 Jan 3;8(1):644-657

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Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase.

Schröder M, Häfner AK, Hofmann B, Rådmark O, Tumulka F, Abele R, Dötsch V, Steinhilber D
Biochimica et biophysica acta 2014 Oct;1842(10):1538-47

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TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Somalinga BR, Day CE, Wei S, Roth MG, Thomas PJ
PloS one 2012;7(4):e35818

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Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential.

Sun W, Guo C, Meng X, Yu Y, Jin Y, Tong D, Geng J, Huang Q, Qi J, Liu A, Guan R, Xu L, Sun D, Ji W, Liu P, Liu F, Sun H, Ji G, Fu S, Bai J
Journal of investigative medicine : the official publication of the American Federation for Clinical Research 2012 Apr;60(4):689-94

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Proteomic analysis of human small cell lung cancer tissues: up-regulation of coactosin-like protein-1.

Jeong HC, Kim GI, Cho SH, Lee KH, Ko JJ, Yang JH, Chung KH
Journal of proteome research 2011 Jan 7;10(1):269-76

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10781-1-AP(COTL1 antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using COTL1 antibody 10781-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-COTL1 (IP:10781-1-AP, 3ug; Detection:10781-1-AP 1:3000) with mouse kidney tissue lysate 12500ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 10781-1-AP(COTL1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 10781-1-AP(COTL1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue