60237-1-IG

antibody from Invitrogen Antibodies

Targeting: COTL1 CLP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

60237-1-IG

Provider

Invitrogen Antibodies

Product name

COTL1 Monoclonal Antibody (4A3D9)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MATKIDKEA CRAAYNLVRD DGSAVIWVTF KYDGSTIVPG EQGAEYQHFI QQCTDDVRLF AFVRFTTGDA MSKRSKFALI TWIGENVSGL QRAKTGTDKT LVKEVVQNFA KEFVISDRKE LEEDFIKSEL KKAGGANYDA QTE (1-142 aa encoded by BC010884)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

4A3D9

Vial size

150 µL

Concentration

0.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-IG ( COTL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (Product # 60237-1-IG, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 60237-1-IG (COTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60237-1-IG ( COTL1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60237-1-IG ( COTL1 Antibody) at dilution of 1:50.