HPA058330

antibody from Atlas Antibodies

Targeting: STAG3

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA058330

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA058330, RRID:AB_2683681

Product name

Anti-STAG3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVTTYC
HSLSPVENTAETSPQVLPSSKRRRVEGPAKPNRED
VSS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and stomach tissues using Anti-STAG3 antibody. Corresponding STAG3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, kidney, stomach and testis using Anti-STAG3 antibody HPA058330 (A) shows similar protein distribution across tissues to independent antibody HPA049106 (B).

Antibody #2 product nr

HPA049106

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-STAG3 antibody HPA058330.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-STAG3 antibody HPA058330.

Sample type

HUMAN