NBP1-86641
antibody from Novus Biologicals
Targeting: SORBS1
CAP, FLJ12406, KIAA1296, ponsin, SH3D5, sh3p12
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
Reference
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- Product number
- NBP1-86641 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86641, RRID:AB_11032588
- Product name
- Rabbit Polyclonal SORBS1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SORBS1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SORBS1 Antibody [NBP1-86641] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Tonsil
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SORBS1 Antibody [NBP1-86641] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human heart muscle shows strong membranous and cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-SORBS1 antibody NBP1-86641 (A) shows similar protein distribution across tissues to independent antibody NBP2-33383 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human testis.